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	Provedor de dados BJMBR (3) Autor Braghirolli,D.I. (3) Pranke,P. (3) Caberlon,B. (1) Cardozo,N.S.M. (1) Dias,M.L. (1) Fernandes,R.A. (1) Gamba,D. (1) Netto,C.A. (1) Paim,A. (1) Petry,JFTC. (1) Steffens,D. (1) Tessaro,I.C. (1) Zanatta,G. (1) Mais... Palavra-chave Cell adhesion (1) Electrospinning (1) Electrospun scaffolds (1) Endothelial progenitor cells (1) Mesenchymal stem cells (1) Nanotechnology (1) Perfusion (1) Poly(trimethylene carbonate-co-L-lactide) (1) Shear stress (1) Smooth muscle cells (1) Stem cell (1) Stem cells (1) Tissue engineering (1) Vascular scaffolds (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2019 (1) 2018 (1) 2012 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Human dental pulp stem cell adhesion and detachment in polycaprolactone electrospun scaffolds under direct perfusion BJMBR Paim,A.; Braghirolli,D.I.; Cardozo,N.S.M.; Pranke,P.; Tessaro,I.C.. Cell adhesion in three-dimensional scaffolds plays a key role in tissue development. However, stem cell behavior in electrospun scaffolds under perfusion is not fully understood. Thus, an investigation was made on the effect of flow rate and shear stress, adhesion time, and seeding density under direct perfusion in polycaprolactone electrospun scaffolds on human dental pulp stem cell detachment. Polycaprolactone scaffolds were electrospun using a solvent mixture of chloroform and methanol. The viable cell number was determined at each tested condition. Cell morphology was analyzed by confocal microscopy after various incubation times for static cell adhesion with a high seeding density. Scanning electron microscopy images were obtained before and after... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cell adhesion; Perfusion; Shear stress; Stem cell; Electrospun scaffolds. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500615 Poly(trimethylene carbonate-co-L-lactide) electrospun scaffolds for use as vascular grafts BJMBR Braghirolli,D.I.; Caberlon,B.; Gamba,D.; Petry,JFTC.; Dias,M.L.; Pranke,P.. Currently, there is great clinical need for suitable synthetic grafts that can be used in vascular diseases. Synthetic grafts have been successfully used in medium and large arteries, however, their use in small diameter vessels is limited and presents a high failure rate. In this context, the aim of this study was to develop tissue engineering scaffolds, using poly(trimethylene carbonate-co-L-lactide) (PTMCLLA), for application as small diameter vascular grafts. For this, copolymers with varying trimethylene carbonate/lactide ratios – 20/80, 30/70, and 40/60 – were submitted to electrospinning and the resulting scaffolds were evaluated in terms of their physicochemical and biological properties. The scaffolds produced with PTMCLLA 20/80, 30/70, and 40/60... Tipo: Info:eu-repo/semantics/article Palavras-chave: Poly(trimethylene carbonate-co-L-lactide); Vascular scaffolds; Mesenchymal stem cells; Endothelial progenitor cells; Smooth muscle cells. Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000800611 Viability of mesenchymal stem cells during electrospinning BJMBR Zanatta,G.; Steffens,D.; Braghirolli,D.I.; Fernandes,R.A.; Netto,C.A.; Pranke,P.. Tissue engineering is a technique by which a live tissue can be re-constructed and one of its main goals is to associate cells with biomaterials. Electrospinning is a technique that facilitates the production of nanofibers and is commonly used to develop fibrous scaffolds to be used in tissue engineering. In the present study, a different approach for cell incorporation into fibrous scaffolds was tested. Mesenchymal stem cells were extracted from the wall of the umbilical cord and mononuclear cells from umbilical cord blood. Cells were re-suspended in a 10% polyvinyl alcohol solution and subjected to electrospinning for 30 min under a voltage of 21 kV. Cell viability was assessed before and after the procedure by exclusion of dead cells using trypan blue... Tipo: Info:eu-repo/semantics/article Palavras-chave: Nanotechnology; Tissue engineering; Electrospinning; Stem cells. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000200006 Registros recuperados: 3 Primeira ... 1 ... Última

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